1. Background
  2. Data Collection Methods
  3. Data Quality Control
  4. Summary

Adapted from Kendall and Riley (1999).

Steps in qPCR

  1. Denature DNA by heating
  2. Anneal primers to single strands of DNA
  3. Synthesize DNA

Synthesize DNA

DNA polymerase is used to synthesize new DNA by adding complementary nucleotides to the denatured, single-stranded DNA template. The optimal temperature for Taq (Thermus aquatius) DNA polymerase is 72 ℃.