1. Background
  2. Data Collection Methods
  3. Data Quality Control
  4. Summary

Adapted from Kendall and Riley (1999).

Steps in qPCR

  1. Denature DNA by heating
  2. Anneal primers to single strand of DNA
  3. Synthesize DNA

Denature DNA

Genomic DNA can be denatured by heating to 95 ℃ to disrupt hydrogen bonds between nucleotide base pairs. This produces two single strands of DNA.