1. Background
  2. Data Collection Methods
  3. Data Quality Control
  4. Summary

Adapted from: Kendall and Riley (1999).

Let's Review

The polymerase chain reaction (PCR) is a laboratory technique that amplifies deoxyribonucleic acid (DNA).

Before proceeding to the review questions, you should feel comfortable with the following:

  1. The main steps in one cycle of a PCR
  2. The difference between PCR and qPCR
  3. Definition of CT and why it is important
  4. PCR efficiency and its importance
  5. Absolute vs. relative quantification
  6. Quality control methods used to ensure data quality