Transcriptomics

Review (3/5)

Sample prepapartion for RNA-Seq analysis includes:

Extracting RNA from a cell(s), fragmenting the RNA into smaller pieces, creating a cDNA library, and adding adapters to cDNA fragments.

Extracting DNA from a cell(s), fragmenting the DNA into smaller pieces, transcribing the DNA to obtain RNA for sequencing.

No sample preparation is needed.

Extracting DNA from a cell, amplifying it by PCR, transcribing the DNA to produce RNA.