1. Background
  2. Data Collection Methods
  3. Data Quality Control
  4. Summary

Key Concepts for Measurement

Fluorometer Calculated Endpoints - Non-photochemical Quenching

(Maxwell and Johnson, 2000)

NPQ = (Fm0-Fm')/Fm'

This is sometimes referred to as SVN is linearly related to heat dissipation (scale = 0 to infinity)

Variables Measured Directly

  1. Fm0 - maximum fluorescence; obtained in the absence of photochemical quenching
  2. Fm' - light-adapted maximum fluorescence; obtained after a saturating pulse of light